Table 2.

Kinetic and affinity constants for NOS3 peptide binding to MAP kinases determined by BLI. Parameters were determined by fitting to a global one‐state binding model. nd, not determined.

Ligand	Kinase	K D (nm)	k on (m −1 s−1)	k off (s−1)
NtMAP	ERK	940	6.6 × 103	6.2 × 10‐2
	p38	31	1.7 × 104	5.3 × 10−4
	JNK	38	1.4 × 104	5.4 × 10−4
p114	ERK	nd	nd	nd
	p38	nd	nd	nd
	JNK	nd	nd	nd
AI	ERK	170	1.2 × 104	2.0 × 10−3
	p38	61	1.6 × 104	9.6 × 10−4
	JNK	3	8.4 × 104	534 × 104
p633	ERK	nd	nd	nd
	p38	nd	nd	nd
	JNK	nd	nd	nd