Table 6.
Concentration of constituents determined in black cohosh root extract sample 5 by standard addition followed by liquid chromatograph coupled with tandem mass spectrometry (LC-MS/MS) analysisa
| Constituent | Percent (w/w) |
|---|---|
| Actein | 0.47 |
| Allocryptonine | <0.01 |
| Caffeic acid | 0.28 |
| Cimifugin | <0.01 |
| Cimicifugoside H-1 | <0.01 |
| Cimiracemodise C | 2.13 |
| 27-Deoxyactein (23-epi-26-deoxyactein) | 2.09 |
| 26-Deoxycimicifugoside | 0.08 |
| Ferulic acid | 0.04 |
| Isoferrulic acid | 0.70 |
| Magnoflorine | 0.01 |
| Prim-O-glucosylcimifugin | <0.01 |
a
Values shown are average of 2 replicates. Percent of constituent in extract is estimated as: weight of constituent/weight of extract*100. The lower limit of quantitation for this method is approximately 0.01% (w/w).