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. 2022 Mar 17;12(5):937–958. doi: 10.1002/2211-5463.13391

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© 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — The effect of EGCG on enzyme expression in the DNL pathway, free fatty acid and ATP levels in HCT116 tumor xenografts of nude mice. (A) Representative image of tumor immunohistochemistry staining (scale bar = 40 μm) shows the expression of enzymes in the DNL pathway. (B) ACLY, ACC and FASN expression was measured using an immunoblotting assay and (C) quantitated in a histogram compared to 100% of the control group. (D) The histogram represents free fatty acid and (E) ATP levels. Representative data were collected from five tumors in each group and presented as the mean ± SD. *P < 0.05 compared to the control using one‐way ANOVA with Tukey’s post‐hoc analysis.