Table 1.
The kinetic constants and production yield of purified Nbs.
| Nanobody | kon(M-1s-1) | koff (s-1) | KD (M) | Production yield (mg/L) |
|---|---|---|---|---|
| NbBotI-01 | 4.87 ± 0.08×106 | 4.64 ± 0.15×10-4 | 9.53 ± 0.35×10-11 | 7.67 |
| NbBotI-17 | 6.52 ± 0.29×106 | 3.08 ± 0.16×10-2 | 4.72 ± 0.32×10-9 | 3.78 |
The production yield in milligrams of purified manobody is given per liter of culture. The association and dissociation rate constants were measured by surface plasmon resonance and used to calculate the equilibrium dissociation constant (KD). +/− Values present the standard error of the kinetic parameters.