TABLE 5.
Quantification of building blocks (% w/w) with heparinase I+II+III digests of BMH affinity fractions.
| BMH | Hep* | LA | HA1 | HA2 | HA3 | HA4 | HA5 | ||
| ΔIVa | 2.1 | 2.3 | 2.1 | 2.0 | 2.0 | 2.0 | 2.0 | ||
| ΔIVsgal | 0.2 | 0.3 | 0.2 | 0.2 | 0.2 | 0.1 | 0.1 | ||
| ΔIVs | 3.7 | 4.1 | 3.2 | 2.9 | 2.5 | 2.5 | 2.2 | ||
| ΔIIa | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.6 | 0.7 | ||
| ΔIIIa | 1.6 | 1.7 | 1.6 | 1.5 | 1.4 | 1.3 | 1.2 | ||
| ΔIIsgal | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | ||
| Unsaturated building blocks | ΔIIs | 7.1 | 7.1 | 7.4 | 7.2 | 6.4 | 6.9 | 6.5 | |
| ΔIIIs | 23.4 | 26.1 | 22.7 | 17.6 | 14.3 | 13.7 | 13.0 | ||
| ΔIa | 0.2 | 0.2 | 0.1 | 0.3 | 0.3 | 0.3 | 0.4 | ||
| ΔIIa-IVsglu | 1.1 | 0.2 | 1.9 | 2.6 | 2.6 | 2.9 | 2.9 | ||
| ΔIIs | 0.1 | 0.0 | 0.1 | 0.2 | 0.3 | 0.3 | 0.4 | ||
| ΔIIIs | 0.9 | 0.9 | 1.3 | 1.0 | 0.9 | 1.0 | 0.9 | ||
| ΔIs | 45.7 | 46.0 | 44.3 | 48.8 | 49.8 | 47.7 | 44.3 | ||
| ΔIIa-IIsglu | 1.1 | 0.5 | 1.5 | 2.0 | 2.4 | 3.0 | 3.6 | ||
| ΔIs | 1.1 | 0.7 | 1.6 | 1.4 | 1.5 | 2.3 | 4.2 | ||
| ΔIs-IdoA(2S) | 0.2 | 0.2 | 0.1 | 0.1 | 0.2 | 0.1 | 0.3 | ||
| ΔIIs-IIsglu | 0.4 | 0.0 | 0.5 | 0.7 | 1.0 | 2.1 | 2.6 | ||
| ΔIa-IIsglu | 0.1 | 0.0 | 0.1 | 0.2 | 0.2 | 0.3 | 0.3 | ||
| ΔIs-IIsglu | 0.9 | 0.1 | 1.2 | 1.8 | 3.7 | 3.1 | 2.4 | ||
|
| |||||||||
| GlcNS | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | ||
| GlcN(NS,3S) | 0.2 | 0.2 | 0.1 | 0.1 | 0.2 | 0.1 | 0.1 | ||
| GlcN(NS,6S) | 0.3 | 0.2 | 0.3 | 0.2 | 0.3 | 0.2 | 0.2 | ||
| NRE | U(2,2,0) | 0.5 | 0.7 | 0.4 | 0.3 | 0.3 | 0.3 | 0.5 | |
| IIs glu | 0.0 | 0.0 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | ||
| Isid | 2.0 | 2.1 | 1.7 | 1.7 | 1.9 | 1.6 | 1.6 | ||
| GlcN(NS,3S,6S) | 0.3 | 0.3 | 0.5 | 0.4 | 0.4 | 0.4 | 0.5 | ||
|
| |||||||||
| Sulfates/Carboxylates | 2.36 | 2.33 | 2.37 | 2.41 | 2.47 | 2.47 | 2.48 | ||
| Sulfates/Carboxylates (sites) | 2.58 | 2.51 | 2.41 | 2.48 | 2.50 | 2.57 | |||
|
|
|||||||||
| NAc (sites) | 20.9 | 22.8 | 25.4 | 22.3 | 22.0 | 22.4 | |||
| NAc | 7.3 | 6.9 | 7.1 | 8.7 | 9.3 | 9.5 | 10.3 | ||
| Heparin | 6-OH | 38.2 | 40.9 | 37.0 | 31.3 | 27.1 | 26.5 | 25.7 | |
| 2-OH | 20.9 | 19.0 | 22.0 | 23.3 | 23.8 | 26.2 | 27.5 | ||
| 3S | 4.6 | 2.6 | 6.2 | 7.0 | 8.6 | 10.4 | 13.4 | ||
|
| |||||||||
| Tetra 3S/Di 3S (mole/mole) | 1.1 | 0.4 | 1.2 | 1.8 | 2.4 | 2.0 | 1.5 | ||
| Sites/Chain | 0.7 | 0.1 | 1.0 | 1.3 | 1.6 | 2.0 | 2.1 | ||
NAc, % N-acetylated glucosamines; 6-OH, % 6-OH glucosamines; 2-OH, % 2-OH uronic acids; 3S, % 3-O-sulfated glucosamines.
HA, high affinity; Hep*, starting heparin; LA, low affinity; BMH, bovine mucosa heparin. Tetra 3S/Di 3S: Ratio of 3S building blocks between tetrasaccharides and disaccharides, Sites/Chain: number of ATIII binding pentasaccharides by chain.