TABLE 6.

Quantification of building blocks (% w/w) with heparinase I+II+III digests of BLH affinity fractions.

	BLH	Hep*	LA	HA1	HA2	HA3	HA4	HA5
	ΔIVa	0.7	0.6	0.8	0.7	0.6	0.6	0.7
	ΔIVsgal	0.0	0.0	0.0	0.0	0.0	0.0	0.0
	ΔIVs	0.5	0.4	0.5	0.4	0.3	0.4	0.4
	ΔIIa	0.3	0.2	0.2	0.2	0.2	0.2	0.3
	ΔIIIa	0.0	0.1	0.1	0.1	0.1	0.1	0.1
	ΔIIsgal	0.0	0.1	0.1	0.1	0.1	0.1	0.1
Unsaturated building blocks	ΔIIs	4.2	4.8	5.8	5.2	4.3	5.0	5.5
	ΔIIIs	4.8	5.1	6.3	4.7	3.8	3.7	4.0
	ΔIa	0.0	0.0	0.0	0.0	0.0	0.0	0.1
	ΔIIa-IVsglu	0.1	0.0	0.2	0.3	0.2	0.3	0.3
	ΔIIs	0.3	0.0	0.2	0.2	0.3	0.7	1.0
	ΔIIIs	0.1	0.0	0.4	0.2	0.1	0.2	0.2
	ΔIs	76.0	78.2	70.5	71.8	69.6	65.3	63.7
	ΔIIa-IIsglu	0.8	0.1	1.4	1.9	1.8	1.9	2.4
	ΔIs	0.9	0.4	1.8	1.0	1.0	1.4	3.1
	ΔIs-IdoA(2S)	0.6	0.7	0.4	0.3	0.5	0.5	0.5
	ΔIIs-IIsglu	0.8	0.0	1.1	1.6	2.8	4.9	5.2
	ΔIa-IIsglu	0.0	0.0	0.1	0.0	0.0	0.0	0.0
	ΔIs-IIsglu	1.3	0.1	1.2	2.8	5.3	4.9	2.8
	GlcNS	0.0	0.0	0.1	0.0	0.0	0.0	0.0
	GlcN(NS,3S)	0.2	0.2	0.2	0.2	0.2	0.2	0.2
	GlcN(NS,6S)	0.3	0.2	0.2	0.2	0.2	0.2	0.2
NRE	U(2,2,0)	0.1	0.2	0.2	0.1	0.1	0.1	0.1
	IIs glu	0.0	0.0	0	0	0	0	0
	Isid	3.7	4.2	2.3	2.4	3.0	2.3	2.2
	GlcN(NS,3S,6S)	0.7	0.7	0.7	0.6	0.7	0.7	0.8
	Sulfates/Carboxylates	2.84	2.84	2.80	2.79	2.83	2.82	2.81
	Sulfates/Carboxylates (sites)	2.84		2.84	2.67	2.81	2.72	2.78
	NAc (sites)	11.2		14.7	15.1	12.8	8.5	10.1
	NAc	2.1	1.6	2.6	2.7	2.5	2.7	3.3
Heparin	6-OH	7.7	9.1	10.1	7.9	7.7	6.6	7.2
	2-OH	10.0	7.7	13.0	13.9	17.6	18.6	19.7
	3S	4.0	1.8	5.1	6.7	8.0	9.9	11.2
	Tetra 3S/Di 3S (mole/mole)	1.3	0.5	1.0	2.8	4.3	3.2	1.5
	Sites/Chain	0.3	0.0	0.6	0.9	1.2	1.6	1.4

NAc, % N-acetylated glucosamines; 6-OH, % 6-OH glucosamines; 2-OH, % 2-OH uronic acids; 3S, % 3-O-sulfated glucosamines.

HA, high affinity; Hep*, starting heparin; LA, low affinity; BLH, bovine lung heparin. Tetra 3S/Di 3S: Ratio of 3S building blocks between tetrasaccharides and disaccharides, Sites/Chain: number of ATIII binding pentasaccharides by chain.