TABLE 9.

Quantification of building blocks (% w/w) with heparinase II digests of BLH affinity fractions.

	BLH	Hep*	LA	HA1	HA2	HA3	HA4	HA5
	ΔIVa	0.1	0.1	0.1	0.1	0.1	0.1	0.1
	ΔIVsgal	0.0	0.0	0.0	0.0	0.0	0.0	0.0
	ΔIVs	0.2	0.3	0.4	0.3	0.2	0.3	0.4
	ΔIIa	0.1	0.1	0.1	0.1	0.1	0.1	0.2
	ΔIIIa	0.1	0.1	0.1	0.1	0.1	0.1	0.1
	ΔIIsgal	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Unsaturated building blocks	ΔIIs	4.8	4.7	5.3	4.9	3.9	4.6	5.2
	ΔIIIs	4.9	5.1	6.4	4.7	3.7	3.7	4.0
	ΔIa	0.1	0.0	0.0	0.0	0.0	0.1	0.1
	ΔIIa-IVsglu	0.1	0.0	0.2	0.3	0.2	0.3	0.3
	ΔIIs	0.0	0.0	0.0	0.0	0.0	0.0	0.0
	ΔIIIs	0.0	0.0	0.0	0.0	0.0	0.0	0.0
	ΔIs	76.1	78.2	71.0	72.4	69.8	66.9	66.0
	ΔIIa-IIsglu	0.8	0.4	1.3	2.0	1.8	1.8	1.7
	ΔIs	0.0	0.1	0.1	0.1	0.0	0.0	0.1
	ΔIs-IdoA(2S)	0.4	0.7	0.4	0.3	0.5	0.5	0.4
	ΔIIs-IIsglu	0.9	0.1	1.2	1.7	2.9	4.8	4.6
	ΔIa-IIsglu		0.0	0.0	0.0	0.0	0.0	0.0
	ΔIIs-Isid	0.2	0.0	0.8	0.3	0.3	0.3	0.4
	ΔIIa-IVsglu-Isid						0.0	0.1
	ΔIs-IIsglu	1.5	0.1	1.5	3.2	6.0	4.9	2.3
	ΔIIa-IIsglu-Isid						0.2	1.1
	ΔIs-Isid	0.5	0.2	0.6	0.4	0.5	0.6	0.7
	ΔIIs-IIsglu-Isid						0.0	0.6
	ΔIs-IIsglu-Isid						0.1	0.7
	GlcNS	0.0	0.0	0.0	0.0	0.0	0.1	0.0
	GlcN(NS,3S)	0.1	0.1	0.1	0.1	0.1	0.1	0.1
	GlcN(NS,6S)	0.2	0.2	0.2	0.2	0.2	0.2	0.2
NRE	U(2,2,0)	0.2	0.2	0.0	0.1	0.1	0.1	0.1
	IIs glu	0.0	0.0	0.0	0.0	0.1	0.1	0.1
	Isid	3.7	4.2	2.3	2.4	3.0	2.4	2.3
	GlcN(NS,3S,6S)	0.6	0.7	0.5	0.5	0.6	0.5	0.5

HA, high affinity; Hep*, starting heparin; LA, low affinity; BLH, bovine lung heparin.