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. 2022 Apr 11;10:879192. doi: 10.3389/fbioe.2022.879192

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Copyright © 2022 Ma, Yin, Hao, Liu, Shi, Diao, Yu, Liu, Chen and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of human umbilical cord mesenchymal stem cells (hucMSCs) and exosomes secreted from human umbilical cord mesenchymal stem cells (hucMSC-Exos). (A) Microscopic images of hucMSCs morphology. Scale bar: 250 μm. (B) Flow cytometry results for expression of stem cell surface markers CD73, CD90, CD105, CD34, CD45, and HLA-DR. (C) Alizarin red, Oil Red O, and Safranine O staining for osteogenic, adipogenic, and chondrogenic differentiation. Scale bar: 50 μm. (D) TEM images of hucMSC-Exos morphology. Scale bar: 100 nm. (E,F) NTA-determined size distribution of hucMSC-Exos. (G) Western blotting showing the expression of the markers (CD9, CD63, CD81, TSG101, HSP70, and Calnexin) of hucMSC-Exos. The lysate of hucMSCs served as the control group. (H) Uptake of hucMSC-Exos by HCECs in vitro. PBS was used as a negative control. Scale bar: 20 μm. (I) Uptake of hucMSC-Exos by cornea in vivo. Scale bar: 50 μm.