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. 2022 Apr 11;10:879192. doi: 10.3389/fbioe.2022.879192

FIGURE 7.

FIGURE 7

Effect of combination treatment with hucMSC-Exos and autophagy regulators on autophagy levels in CI mouse corneas. (A) Representative high-magnification TEM images showing the autophagosome counts in the groups. Scale bar: 500 and 100 nm. (B) Western blotting showing autophagy markers p62, Beclin-1, and LC3B. (C) Western blotting showing the AMPK-mTOR-ULK1 autophagy flux pathway proteins pAMPK, AMPK, pULK1, ULK1, pmTOR and mTOR. (D) Relative band densities of p62, Beclin-1, LC3B II/I, pAMPK/AMPK, pULK1/ULK1, and pmTOR/mTOR, quantified in bar graphs. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (Control: normal corneas treated with PBS, CI + PBS: injured corneas treated with PBS, CI + Exo: injured corneas treated with 1 × 106/μL hucMSC-Exos, CI + Exo + AA: injured corneas treated with 1 × 106/μL hucMSC-Exos and 10 μM Rapamycin, CI + Exo + AI: injured corneas treated with 1 × 106/μl hucMSC-Exos and 50 μM Compound C).