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. 2022 Apr 11;10:879192. doi: 10.3389/fbioe.2022.879192

FIGURE 8.

FIGURE 8

Effect of combination treatment with hucMSC-Exos and autophagy regulators on apoptosis and inflammation in CI mice. (A,B) Mouse eyeballs were harvested at 48 h post-injury. Representative TUNEL staining images of apoptotic cells. Positive incidences are shown in the bar graph. Scale bar: 50 μm. (C,D) Western blotting showing the apoptosis-related proteins Bcl-2, Bax, and cleaved Caspase-3. The relative band densities were quantified using bar graphs. (E) Quantitative PCR results for mRNA levels of the pro-inflammatory factors TNF-α, IL-1β, IL-6, and CXCL-2, and quantified in bar graphs. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (Control: normal corneas treated with PBS, CI + PBS: injured corneas treated with PBS, CI + Exo: injured corneas treated with 1 × 106/μl hucMSC-Exos, CI + Exo + AA: injured corneas treated with 1 × 106/μl hucMSC-Exos and 10 μM Rapamycin, CI + Exo + AI: injured corneas treated with 1 × 106/μl hucMSC-Exos and 50 μM Compound C).