TABLE 1.
Sequence of primers for qPCR.
| Gene | Species | Direction | Primer sequence 5'→3' |
|---|---|---|---|
| Cyclin A | Human | Forward | AACTTCAGCTTGTGGGCACT |
| Reverse | CTGGTGGGTTGAGGAGAGAA | ||
| Cyclin E | Human | Forward | CCATCCTTCTCCACCAAAGA |
| Reverse | AGCACCTTCCATAGCAGCAT | ||
| CDK2 | Human | Forward | CCAGGAGTTACTTCTATGCCTGA |
| Reverse | TTCATCCAGGGGAGGTACAAC | ||
| PCNA | Human | Forward | GCCAGAGCTCTTCCCTTACG |
| Reverse | TAGCTGGTTTCGGCTTCAGG | ||
| GAPDH | Human | Forward | AAGAAGGTGGTGAAGCAGGC |
| Reverse | TCCACCACCCTGTTGCTGTA | ||
| TNF- α | Mouse | Forward | ACCCTCACACTCAGATCATCTT |
| Reverse | GGTTGTCTTTGAGATCCATGC | ||
| IL-1β | Mouse | Forward | TGCCACCTTTTGACAGTGATG |
| Reverse | TGATGTGCTGCTGCGAGATT | ||
| IL-6 | Mouse | Forward | TGATGGATGCTACCAAACTGGA |
| Reverse | TGTGACTCCAGCTTATCTCTTGG | ||
| CXCL-2 | Mouse | Forward | AAGGCAAGGCTAACTGACCTG |
| Reverse | TTGGTTCTTCCGTTGAGGGAC | ||
| β-actin | Mouse | Forward | GATTACTGCTCTGGCTCCTAGC |
| Reverse | GACTCATCGTACTCCTGCTTGC |