Primer sequences for RT-PCR amplification.
Sense | Antisense | |
---|---|---|
IL-1 | 5′-GGAAGGAACCATCTCACTGT-3′ | 5′-CAGTGTGGTCCACTCTCAAC-3′ |
IL-6 | 5′-CAATGAGGAGACTTGCCTGG-3′ | 5′-TGGACTGCAGGAACTCCTTA-3′ |
TNF-α | 5′-TTCTGCCTGCTGCACTTTATG-3′ | 5′-TGGGCTACAGGCTTTGTCAT-3′ |
NF-κB | 5′-CCTGCTGGCTGTGAGGAATAC-3′ | 5′-ACTTCTGCTCTGACACCTCCC-3′ |
β-actin | 5′-CCTGGATACCGCAGCTAGGA-3′ | 5′-GCGGCGCAATACGAACCCC-3′ |