Table 2. Affinity of proteins for polysaccharides determined by ITC.
| Protein | K d | ΔG (kcal mol−1) | ΔH (kcal mol−1) | TΔS (kcal mol−1) | n † |
|---|---|---|---|---|---|
| iMtGlu5 | (5.71 ± 0.44) × 10−6 | −7.15 | −18.3 | −11.2 | 1.00 |
| iΔCBM | NB‡ | — | — | — | — |
| gMtCBM | (4.50 ± 0.27) × 10−2 | −1.83 | −16.2 | −14.4 | 0.94 |
| GST | NB | — | — | — | — |
| iTmCel5A§ | NB | — | — | — | — |
| iTmCel5A-CBM | (8.20 ± 2.05) × 10−6 | −6.94 | −17.5 | −10.5 | 1.00 |
Number of binding sites on the protein.
No binding detected.
Binding too weak to quantify by ITC.