Fig. 4.
(A–D) Hematoxylin and eosin staining of adult murine intestinal organoids cultured in eECM matrices of varying RGD concentration and mechanical stiffness under the air–liquid interface configuration for 3 days. (E) MMP activity normalized to average organoid counts was found to increase with increasing matrix stiffness. (F) Inhibition of enzymatic degradation had an increasingly negative impact on organoid formation within stiffer matrices. All data is shown as mean ± SEM. *indicates statistical significance (Student’s t-test, p < 0.05).