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| Target gene | Sequences | |
|---|---|---|
| Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | |
| ALP | TACACGGACACTGAGATGCGCT | TCGTGCATTATCTGATAGGTGA |
| BMP-2 | GCTAAACTTGACGACGCTCGT | CTGCTCGGTTCCCGTTA |
| Col-I | CTAAAGAATGACTACAGCTA | TCCACCCCTAGTGCGTTGCT |
| OCN | CTGTTTAGGGTGTGTGTCGC | TAGCGAGGCAACGAGATCAA |
| OPN | TCTGGAGAGGAGTGAAGCTC | AGGGAAGCGTCAATCTTAAG |
| RUNX2 | CTGTCCCTCCCTCACCCCGT | GACGCCTGCTGTCTTCTGTAG |
| GAPDH (IC) | CCTCCAGGAACTCTCCTCAG | GTATGAGAGCGCAGCCAACGG |
