Secondary structure components of proteins (5.0 μM) reaction with bixin at pH = 7.4 assessed by Jasco Spectra Manager II Software.
| Samples | Secondary structure components (%) | |||
|---|---|---|---|---|
| α-Helix (±2%) | β-Sheet (±1%) | Turn (±1%) | Random (±2%) | |
| Free HSA | 55.4 | 7.9 | 13.5 | 23.2 |
| HSA + bixin (1 : 2) | 59.1 | 7.3 | 11.9 | 21.7 |
| HSA + bixin (1 : 4) | 63.5 | 6.6 | 10.1 | 19.8 |
| Free AGP | 24.7 | 38.9 | 10.5 | 25.9 |
| AGP + bixin (1 : 2) | 26.4 | 38.2 | 9.8 | 25.6 |
| AGP + bixin (1 : 4) | 28.5 | 39.1 | 8.9 | 23.5 |