The kinetic parameters of the cephradine hydrolase and its variants predicted by PRODA. The data is taken from the ESI of the ref. 30. Reaction conditions are: pH 7.0 at 22 °C.
| Variants | Mutations | k cat (×10−3 s−1) | K m (mM) | k cat/Km (×10−3 M−1 s−1) |
|---|---|---|---|---|
| WT | — | 0.61 | 33.90 | 18.11 |
| Q1 | F261T | 2.33 | 12.10 | 192.72 |
| Q2 | Q55R | 0.02 | 25.16 | 0.61 |
| Q3 | F408L | 0.65 | 70.03 | 9.26 |
| Q4 | P150E | 2.11 | 151.34 | 13.93 |
| Q5 | F261T/F408L | 2.32 | 198.12 | 11.68 |
| Q6 | F261T/P150E | 0.44 | 51.16 | 8.63 |
| Q7 | F261T/L407Q | 0.04 | 7.46 | 5.61 |
| Q8 | F261T/L407H | 0.32 | 77.98 | 4.16 |
| Q9 | F261T/F408Y | 0.19 | 19.47 | 9.84 |
| Q10 | F261T/L407Q/F408Y | 0.26 | 23.68 | 10.99 |
| Q11 | F261T/L407H/F408Y | 0.07 | 5.02 | 13.41 |