Fig 2.

Trafficking (dots per cell) of pHrodo-labeled HER3-DXd in MDA-MB-231 cells transduced with HER3^WT, HER3 mutations, or HER3^EV in the absence (A) or presence (B) of HER2 overexpression. The lysosomal trafficking of 0.1, 1, and 10 nM HER3-DXd into the HER3 transfectants was determined using HER3-DXd labeled with pHrodo. Fluorescence emission images were then collected at 30-minute intervals for up to 12 hours. The level of lysosomal trafficking was expressed as the number of dots per cell. Abbreviations: EV = empty vector, HER = human epidermal growth factor receptor, WT = wild type.