Fig. 5. (A) AO/PI staining of neuronal cells on (a) PG11, (b) PG12 and (c) PG14 on day 11. (B) AO/PI staining of stem cells on (a) PG11, (b) PG12 and (c) PG14 on day 5. The images show that the cells are stained with green due to the binding of acridine orange to the nucleic acid of live cells. Penetration of cells into the 3D matrix is also observed. The images were taken at a magnification of 20× using Leica DM 2500.
