Fig. 3. Identification of Txnrd1 as the APAP potential toxicity target. (A) Western blotting validation of the APAP-P1 target TrxR1 in cell lysates and in cells after performing a pull-down assay. (B) Binding mode of APAP-P1 in the 3D structure of TrxR1. (C) Ser-22, Asp-42, Thr-58, Cys-64, and Gly-132 may be the key residues in the interaction between APAP-P1 and TrxR1.
