Figure 4. Antifungal activity of recombinant DEFB118. Overnight cultures of C. albicans IFO1385 and IFM48311, and C. glabrata IFM5678 and HCG1 were harvested, washed, and suspended in NaPi. Each Candida suspension was inoculated in NaPi with the indicated dilution of recombinant DEFB118 and incubated for two hours at 37°C. The mixtures were then separately plated in agar and incubated at 37°C for 48 hours. The total number of Candida colonies in each plate was counted, then, the percentage of surviving Candida as compared to total colony numbers in the control plates ( Candida with Napi as indicated, 0 mg/mL), was determined. Values are shown as the mean ± SD of three independent experiments. *Significantly different from 0 mg/mL (Dunnett’s test, p<0.05).
