Fig. 2. DMR response of ligands on CHO-FFA4 cells. (A) Real-time DMR response of 100 μM ALA, 10 μM TUG891, 10 μM GSK137647A, 12.5 μM GW9508 and 10 μM TAK875. (B) Real-time DMR of 25 μM ALA after CHO-FFA4 cells pretreated with AH7614 at indicated concentrations. (C) Real-time DMR of ALA at indicated concentrations on the parental cell line CHO-K1. (D) Real-time DMR response of 0.5 μM GSK137647A after CHO-FFA4 cells pretreated with AH7614 at indicated concentrations. (E) Real-time DMR response of 0.5 μM TUG891 after CHO-FFA4 cells pretreated with AH7614 at indicated concentrations. (F) Real-time DMR of GSK137647A at indicated concentrations on CHO-K1 cells. (G) Real-time DMR of 25 μM GW9508 on CHO-FFA4 cells after cells pretreated with AH7614 at indicated concentrations. (H) Amplitudes as a function of each agonist doses. (I) The concentration-dependent inhibition of the 0.5 μM TUG891 by GW9508, GSK137647A, TUG891 and ALA. All data represent mean ± s.d. from three independent measurements, each in duplicate (n = 6).