Fig. 3. DMR response of ligands in CHO-FFA1 cells. (A) Real-time DMR response of 100 μM ALA, 10 μM TAK875, 10 μM GW9508, 25 μM TUG891 and 100 μM GSK137647A. (B) Real-time DMR of 30 μM ALA after CHO-FFA1 cells pretreated with GW1100 at indicated concentrations. (C–E) Real-time DMR response of agonists (0.5 μM TAK875, 1 μM GW9508 and 30 μM TUG891) after cells pretreated with GW1100 at indicated concentrations. (F) Amplitudes as a function of each agonist doses. (G) The concentration-dependent inhibition of the 1 μM GW9508 DMR by TAK875, GW9508, TUG891 and ALA. (H) Amplitudes as a function of MEDICA16 and ALA doses. (I) Real-time DMR response of 25 μM MEDICA16 after cells pretreated with GW1100 at indicated concentrations. All data represent mean ± s.d. from three independent measurements, each in duplicate (n = 6).