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. 2022 Apr 14;11:e70664. doi: 10.7554/eLife.70664

Figure 5. The Clstn3 KO decreases spontaneous inhibitory synaptic ‘mini’ events in Purkinje cells.

(A–C) The Clstn3 KO decreases the frequency but not the amplitude of mIPSCs (A, representative traces; B, left, cumulative probability plot of the mIPSC amplitude right, average amplitude; C, left, cumulative probability plot of the mIPSC inter-event interval right, average frequency). (D) The Clstn3 KO increases the rise but not decay times of mIPSCs. (E) Plot of the number of mIPSC events vs. amplitude exhibiting a normal distribution. (F–H) The Clstn3 KO similarly impairs mIPSCs with a larger ( > 60 pA) and a smaller amplitude ( < 60 pA), which in Purkinje cells are likely generated primarily by basket cell and stellate cell synapses, respectively (F & G, summary graphs for the mIPSC amplitude [F] and frequency [G] separately analyzed for high- and low-amplitude events; H, mIPSC rise [left] and decay times [right], separately analyzed for high- and low-amplitude events). All summary data are means ± SEM. Numbers of cells/mice analyzed are indicated in bar graphs. Statistical analyses were performed using unpaired t-tests (bar graphs with two groups) or Kolmogorov-Smirnov test (cumulative analysis), with *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 5.

Figure 5—figure supplement 1. The capacitance and membrane resistance of Purkinje cells are unaffected by the Clstn3 KO.

Figure 5—figure supplement 1.

(A–E) Summary graphs of the capacitance (left) and input membrane resistance (right) of Purkinje cells determined during the recordings described in Figures 5, 6, 9 and 10. All data are means ± SEM. Statistical analyses were done with unpaired t-test, p > 0.05.
Figure 5—figure supplement 2. Analysis of the kinetics of large ( > 60 pA) and smaller ( < 60 pA) mIPSCs confirm that larger mIPSCs, which are presumably generated by basket-cell synapses closer to the soma, have faster rise but slower decay times.

Figure 5—figure supplement 2.

(A & B) Rise (A) and decay times (B) of large and smaller mIPSCs examined in the same Purkinje cells. All data are means ± SEM. Statistical analyses were performed with paired t-test, ***p < 0.001.