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. 2022 Apr 14;11:e70664. doi: 10.7554/eLife.70664

Figure 6. The Clstn3 KO decreases evoked inhibitory synaptic responses in Purkinje cells.

Figure 6.

(A) Experimental design for recordings of IPSCs evoked by stimulation of basket cell axons (ML, molecular layer; PCL, Purkinje cell layer; GCL, granule cell layer; PC, Purkinje cell; Rec., recording patch pipette). (B & C) The Clstn3 KO decreases the amplitude of evoked basket-cell IPSCs (B, representative traces of pairs of evoked IPSCs with a 50ms inter-stimulus interval; (C) summary graphs of the amplitude of the first IPSC). (D & E) The Clstn3 KO in Purkinje cells does not affect the release probability at inhibitory synapses as judged by the coefficient of variation (D) and the paired-pulse ratio with an interstimulus interval of 50ms (E) of evoked IPSCs. (F) The Clstn3 KO in Purkinje cells has no significant effect on IPSC kinetics (left, rise times; right, decay times of evoked ISPCs). All summary data are means ± SEM. Numbers of cells/mice analyzed are indicated in bar graphs. Statistical analyses were performed using unpaired t-tests, with *p < 0.05.