Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent(Mus musculus) | Constitutive Cas9 | PMID:25263330 | JAX ID: 024858 | |
| Genetic reagent(Mus musculus) | Pcp2-Cre | PMID:11105049 | JAX ID:004146 | |
| Genetic reagent(Mus musculus) | RiboTag | PMID:19666516 | JAX ID:029977 | |
| Cell line(Homo sapiens) | HEK293T | ATCC | CRL-11268 | |
| Recombinant DNA reagent | AAV-U6-sg66-U6-sg21-CAG tdTomato | This paper | Sg66 and sg21 were cloned in an AAV backbone and made into the AAVDJ serotype. See sgRNA design and generation of Vectors for cloning details, Virus production for how the AAVs were produced | |
| Recombinant DNA reagent | Lentiviral sg66 and sg21-CAG tdTomato | This paper | Sg66 and sg21 were cloned into a lentiviral shuttle plasmid for sparse infection. See Virus production for cloning details and how lentiviruses were produced. | |
| Sequence-based reagent | Clstn1 RNA FISH probe | Advanced Cell Diagnostics | Cat: 542611 | |
| Sequence-based reagent | Clstn2 RNA FISH probe | Advanced Cell Diagnostics | Cat: 542621 | |
| Sequence-based reagent | Clstn3 RNA FISH probe | Advanced Cell Diagnostics | Cat: 542631 | |
| Sequence-based reagent | Clstn3 qPCR primers and probe | This paper | See Quantitative RT-PCR and Figure 2—figure supplement 1A for how primers and probe were designed | |
| Antibody | Anti-Clstn3(rabbit polyclonal) | PMID:24613359 | Primary antibody, (1:1000) IB | |
| Antibody | Anti-Actb(mouse monoclonal) | Sigma | #A1978 | Primary antibody, (1:10000) IB |
| Antibody | Anti-vGluT1(rabbit polyclonal) | Yenzym | YZ6089 | Primary antibody, (1:1000) IHC |
| Antibody | Anti-vGluT2(rabbit polyclonal) | Yenzym | YZ6097 | Primary antibody, (1:1000) IHC |
| Antibody | Anti-vGAT(guinea pig polyclonal) | Sysy | 131004 | Primary antibody, (1:1000) IHC |
| Antibody | Anti-GluA1(rabbit polyclonal) | Millipore | AB1504 | Primary antibody, (1:1000) IHC |
| Antibody | Anti-GluA2(mouse monoclonal) | Millipore | MAB397 | Primary antibody, (1:1000) IHC |
| Antibody | Anti-GABA(A)Rα1(mouse monoclonal) | Neuromab | N95/35 | Primary antibody, (1:1000) IHC |
| Chemical compound, drug | Tribromoethanol | Sigma | T48402 | 250 mg/kg for anesthesia |
| Chemical compound, drug | Picrotoxin | Tocris | 1128 | |
| Chemical compound, drug | APV | Tocris | 0106 | |
| Chemical compound, drug | CNQX | Tocris | 1045 | |
| Chemical compound, drug | NBQX | Tocris | 1044 | |
| Chemical compound, drug | QX314 | Tocris | 1014 | |
| Chemical compound, drug | Tetrodotoxin | Cayman Chemical | 14964 | |
| Chemical compound, drug | DAPI | Sigma | D8417 | |
| Chemical compound, drug | Biocytin | Sigma | B4261 | |
| Chemical compund, drug | Pepsin | DAKO | S3002 | 1 mg/ml |
| Sequence-based reagent | Clstn1 | IDT | Mm.PT.58.6236597 | commercially designed |
| Sequence-based reagent | Clstn2 | IDT | Mm.PT.58.6443231 | commercially designed |
| Sequence-based reagent | Clstn3 | IDT | Mm.PT.58.45847813.g | commercially designed |
| Sequence-based reagent | Gapdh | Applied Biosystems | 4352932E | commercially designed |
| Software, algorithm | SnapGene | GSL Biotech | previously existing | |
| Software, algorithm | Image Studio Lite | LI-COR | previously existing | |
| Software, algorithm | pClamp10 | Molecular Device | previously existing | |
| Software, algorithm | Clampfit10 | Molecular Device | previously existing | |
| Software, algorithm | NIS-ElementsAR | Nikon | previously existing | |
| Software, algorithm | ImageJ | NIH | previously existing | |
| Software, algorithm | Neurolucida360 | MBF science | previously existing | |
| Software, algorithm | Adobe Illustrator | Adobe | previously existing | |
| Software, algorithm | GraphpadPrism 8.0 | Graphpad software | previously existing |
IB: immunoblotting, IHC: immunohistochemistry.