Fig. 4. Optimization of different conditions for the detection of NSE. (a) The relationship between the amounts of Apt-5 and the CL intensity. (b) The relationship between the concentration of NSE and the CL intensity. (c) The relationship between concentration of primary mouse anti-NSE antibody and CL intensity. (d) The relationship between concentration of secondary goat antimouse antibody labeled with ALP and CL intensity.
