Fig. 7. Selectivity assays of YFP-MetQ-R189CouA-H88F against 20 natural AAs and d-Met. Changes in FRET ratio were calculated by the method described in Fig. 4. Each data point represents the average of three independent experiments. Assay conditions: 100 nM sensor protein, 50 mM phosphate buffer (pH 9.0), 50 mM NaCl, and 50 μM AA.
