Fig. 1. Purification and characterization of recombinant human UBE2C expressed in E. coli BL21 (DE3). (A) SDS-PAGE showing purified his-tag UBE2C protein in lane 1 and protein molecular weight marker in lane 2. (B) Western blot of purified his-UBE2C expressed in E. coli BL21 (DE3), and (C) western blot of UBE2C expressed in MCF-7 cell lysate. In western blots, proteins were detected with polyclonal rabbit UBE2C antibody followed by anti-rabbit HRP conjugated antibody. (D) Indirect ELISA with 1 : 1000 dilution of polyclonal rabbit UBE2C antibody. Bar diagram represents mean absorption value (at 370 nm) value. Error bars represent the standard deviations.