Fig. 3. MiR-486 silencing repressed the inflammatory response in LPS-stimulated macrophages. Macrophages were transfected with anti-miR-486 or anti-miR-NC prior to LPS stimulation, followed by the detection of miR-486 expression by qRT-PCR assay (A), the mRNA levels of TNF-α, IL-6 and IL-1β by qRT-PCR assay (B), TNF-α (C), IL-6 (D) and IL-1β (E) levels by ELISA. *P < 0.05 vs. control or LPS + anti-miR-NC.
