Fig. 4. SIRT1 was a direct target of miR-486. (A) Nucleotide resolution of the predicted miR-486 binding site in 3′-UTR of SIRT1 mRNA: seed sequence and the mutated seed sequence. (B) Dual-luciferase reporter assay was performed to verify whether SIRT1 was a target of miR-486 by cotransfecting SIRT1-Wt or SIRT1-Mut into macrophages and miR-486 mimics or miR-NC mimics. Macrophages were transfected with miR-NC mimics, miR-486 mimics, anti-miR-NC or anti-miR-486, followed by the detection of SIRT1 mRNA (C) and protein (D) levels. *P < 0.05 vs. miR-NC mimics or anti-miR-NC.