Fig. 5. SIRT1 affected inflammatory response in LPS-stimulated macrophages. Macrophages were transfected with Vector or Vector-SIRT1 and then were stimulated with 1 μg mL⁻¹ of LPS or PBS, followed by the determination of SIRT1 protein expression by Western blot (A and B), the mRNA levels of TNF-α, IL-6 and IL-1β by qRT-PCR assay (C), TNF-α (D), IL-6 (E) and IL-1β (F) levels by ELISA. *P < 0.05 vs. control or LPS + vector.