Fig. 6. SIRT1 moderated the effect of miR-486 on inflammatory response in LPS-stimulated macrophages. Macrophages were transfected with anti-miR-486 or anti-miR-NC and then were treated with 10 μM of EX527 or vehicle, followed by stimulation with LPS. (A–C) After 6 h stimulation, the TNF-α, IL-6 and IL-1β mRNA levels were detected by qRT-PCR assay. (D–F) After 24 h stimulation, the TNF-α, IL-6 and IL-1β levels were determined by ELISA. *P < 0.05 vs. anti-miR-NC or anti-miR-486 + vehicle.
