Fig. 3. Depicts the growth pattern of R. leguminosarum strain RP1 grown in minimal medium supplemented with increasing concentrations of kitazin as a function of time of incubation at 28 ± 2 °C. Data represent the mean ± S. D of three independent experiments done in triplicate (A). Control and kitazin treated SEM micrograph of strain RP1 (red coloured arrows indicate the damage/rupture in cells after exposure to kitazin (A)). CLSM images of live/dead cells of R. leguminosarum strain RP1 stained with AO/PI indicating the cell viability (A) control (B–D) treated with 600, 1200 and 1800 μg mL⁻¹ kitazin (B).