Effect of various concentrations of kitazin on plant growth promoting (PGP) traits of Rhizobium leguminosarum strain RP1a.
| Treatments | Dose rate (μg mL−1) | IAAb (μg mL−1) | P-solubilization (μg mL−1) | EPS (μg mL−1) | ACCc deaminase activity (μM α-ketobutyrate per mg per protein per hour) | Siderophores | HCNg | NH3h | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Phenolate (μg mL−1) | CASf agar (mm) | FeCl3 test | |||||||||
| S. A.d | 2,3-DHBAe | ||||||||||
| Control | 0 | 80.5a ± 2.5 | 31.5a ± 1.8 | 29.5a + 2.6 | 51a ± 3.2 | 54a ± 7.3 | 31.9a ± 2.7 | 18a ± 11 | ++ | ++ | ++ |
| Kitazin | 600* | 77.4a ± 3.4 | 25.4b ± 0.9 | 22.3b + 2.1 | 48.2a ± 6.8 | 38.9b ± 1.7 | 22.3b ± 0.9 | 17ab ± 1 | + | + | + |
| 1200** | 70.2b ± 1.8 | 20.6c ± 0.6 | 18.8c + 1.2 | 44.8ab ± 5.4 | 31.9b ± 1.7 | 14.5c ± 0.7 | 15.6b ± 0.5 | + | + | + | |
| 1800*** | 55.6c ± 2.9 | 15.6d ± 0.5 | 11.6d + 0.6 | 36.2c ± 2.4 | 21.9c ± 0.1.6 | 11.3d ± 0.6 | 13.6c ± 0.5 | − | − | − | |
| Mean value | — | 70.9 | 23.2 | 20.5 | 45.02 | 36.6 | 20 | 16.05 | − | − | − |
| LSD | — | 5.55 | 2.36 | 2.633 | 9.49 | 8.19 | 2.41 | 0.66 | − | − | − |
| F value (treatments) | 45.06 | 90.6 | 19.34 | 5.2 | 34.2 | 107.9 | 15.79 | ||||
a
Each value is a mean of three replicates. Mean values (±) are significant of p ≤ 0.05. Means followed by similar alphabets are not significantly different from each other according to DMRT test. *, ** and *** represents the 1×, 2× and 3× concentrations of kitazin, respectively.
b
Indole acetic acid.
c
1-Amino cyclopropane carboxylate.
d
Salicylic acid.
e
2,3-Dihydroxy benzoic acid.
f
Chrome azurol S agar.
g
Hydrogen cyanide.
h
Ammonia.