. 2019 May 31;9(30):16929–16947. doi: 10.1039/c9ra01253b

Inoculation effect of fungicide tolerant Rhizobium leguminosarum strain RP1 on photosynthetic pigments and proline content of P. sativum plants grown in sandy clay soil supplemented with different doses of kitazin^a.

Treatments	Dose rate (μg per kg per soil)	Photosynthetic pigments (mg g⁻¹ fw)				Proline content (mg g⁻¹ fw)
Treatments	Dose rate (μg per kg per soil)	chl a	chl b	Total chl	Carotenoid	Shoot (90 DAS)	Root (90 DAS)	Leaves (90 DAS)	Grains (130 DAS)
Uninoculated	0	0.31^bc±0.02	0.17^b ± 0.01	0.44^b ± 0.02	1.18^c ± 0.1	7.5^g ± 1.0	20^e ± 2.8	14.6^f ± 1.9	3.4^e ± 1.1
	96*	0.29^d ± 0.02	0.16^d ± 0.0	0.42^cd ± 0.01	1.13^c ± 0.07	12.3^e ± 1.1	26.1^d ± 0.9	18^e ± 0.17	6.8^c ± 0.9
	192**	0.25^f ± 00	0.14^f ± 0.0	0.39^e ± 0.01	1.07e±0.06	17.3^c ± 0.9	33.5^b ± 1.4	22.5^c ± 1.3	9.3^b ± 0.6
	288***	0.22^g ± 0.01	0.14^f ± 0.0	0.37^f ± 0.01	0.98^f ± 0.1	27.8^a ± 3.7	42.3^a ± 1.6	29.8^a ± 1.2	12^a ± 1.0
Inoculated	0	0.40^a ± 0.01	0.22^a ± 0.01	0.47^a ± 0.2	1.48^a ± 0.04	5.7^h ± 1.5	11.2^f ± 1.9	10.2^g ± 2.3	2.3^f ± 0.5
	96*	0.32^b ± 0.03	0.18^b ± 0.01	0.46^a ± 0.07	1.31^b ± 0.2	9.6^f ± 1.2	21.2^e ± 1.6	15.6^f ± 0.5	5.4^d ± 0.5
	192**	0.26^e ± 0.02	0.17^c ± 0.02	0.43^c ± 0.05	1.11^d ± 0.1	15.8^d ± 1.5	27.5^c ± 1.9	20^cd ± 1.0	6.0^c ± 00
	288***	0.24^f ± 00	0.15^e ± 0.01	0.38^f ± 0.02	1.02^e ± 0.1	25.8^b ± 1.9	41.2^a ± 2.6	27.3^b ± 1.5	10.6^b ± 1.5
Mean value	—	0.28	0.16	0.42	1.16	15.2	27.8	19.7	6.9
LSD (p ≤ 0.05)		0.033	0.045	0.068	0.15	3.14	3.18	2.53	1.63
F value un inoculated (df = 1)		23.28	2.42	3.27	15.34	7.48	49.19	25.24	21.27
Inoculated (df = 3)		54.32	1.58	5.28	16.64	141.57	221.55	136.30	84.93
Un inoculated × inoculated (df = 3)		5.47	0.77	0.14	3.03	0.10	4.76	0.66	1.93
P value un inoculated (df = 1)		0.000	0.142	0.092	0.002	0.016	0.000	0.000	0.000
Inoculated (df = 3)		0.000	0.239	0.012	0.000	0.000	0.000	0.000	0.000
Un inoculated × inoculated (df = 3)		0.011	0.028	0.931	0.065	0.959	0.017	0.59	0.171

Each value is a mean of three replicates where each replicate constituted three plants/pot. Mean values are significant at p ≤ 0.05. Means followed by similar alphabets are not significantly different from each other according to DMRT test. *, ** and *** represents the normal recommended (1×), two times more (2×) than recommended dose and three times more (3×) than recommended doses of kitazin.

Inoculation effect of fungicide tolerant Rhizobium leguminosarum strain RP1 on photosynthetic pigments and proline content of P. sativum plants grown in sandy clay soil supplemented with different doses of kitazina.

Inoculation effect of fungicide tolerant Rhizobium leguminosarum strain RP1 on photosynthetic pigments and proline content of P. sativum plants grown in sandy clay soil supplemented with different doses of kitazin^a.