TABLE 5.
Detection of genes sulI, sulII, and bla by PCR and DNA-DNA hybridizationa
| Strain |
sulI
|
sulII
|
bla
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| PCR detection | Plasmid size (kb) | Presence of pSCV | PCR detection | Plasmid size (kb) | Presence of pSCV | PCR detection | Plasmid size (kb) | Presence of pSCV | |
| OU7085 | − | + | 6 | No | − | ||||
| OU7518 | + | 136 | Yes | − | + | 136 | Yes | ||
| OU7519 | − | − | − | ||||||
| OU7520 | + | 140 | Yes | − | + | 140 | Yes | ||
| OU7521 | − | − | + | 90 | No | ||||
| OU7522 | − | − | − | ||||||
| OU7523 | − | − | − | ||||||
| OU7524 | + | 136 | Yes | − | + | 136 | Yes | ||
| OU7525 | − | − | − | ||||||
| OU7526 | + | 90 | No | − | + | 90 | No | ||
| OU7527 | − | + | 6 | No | − | ||||
| OU7528 | − | − | − | ||||||
| OU7529 | + | 90 | No | − | + | 90 | No | ||
| OU7531 | + | 90 | No | − | + | 90 | No | ||
a
PCR results are the same as those for DNA-DNA hybridization. The results of PCR and DNA-DNA hybridization were consistently in agreement.