Table 1.
List of primers used in the study.
| Antibiotic | Gene | DNA sequence (5'-3') | Annealing temperature (°C) | Product (bp) | Reference |
|---|---|---|---|---|---|
| Gentamicin | aph(6)-Id | F: ATCGTCAAGGGATTGAAACC R: GGATCGTAGAACATATTGGC |
50 | 509 | [25] |
| aph(3')-Ia | F: ATGGGCTCGCGATAATGTCG R: AGAAAAACTCATCGAGCATC |
60 | 734 | [23] | |
| aac(3)-IV | F: CTTCAGGATGGCAAGTTGGT R: TCATCTCGTTCTCCGCTCAT |
55 | 286 | [28] | |
| Ampicillin | bla SHV | F: TCGCCTGTGTATTATCTCCC R: CGCAGATAAATCACCACAATG |
52 | 768 | |
| blaCMY | F: TGGCCAGAACTGACAGGCAAA R: TTTCTCCTGAACGTGGCTGGC |
47 | 462 | ||
| blaTEM-1B | F: TCCTTGAGAGTTTTCGCCCC R: TGACTCCCCGTCGTGTAGAT |
60 | 634 | This study | |
| Sulfamethoxazole | sul1 | F: TTCGGCATTCTGAATCTCAC R: ATGATCTAACCCTCGGTCTC |
47 | 822 | [28] |
| Sul2 | F: CACATTGCGGCGTTCTTTGA R: TTGCGGTTTCTTTTAGCGCC |
60 | 241 | This study | |
| Sul3 | F: AGTGGGCGTTGTGGAAGAAA R: AGTAGCTGCACCAATTCGCT |
60 | 361 | ||
| Trimethoprim | dfrA1 | F: GGAGTGCCAAAGGTGAACAGC R: GAGGCGAAGTCTTGGGTAAAAAC |
45 | 367 | [27] |
| dfrA17 | F: GGCGTAATCGGTAGTGGTCC R: CCCCCGCCAGAGACATATAC |
60 | 263 | This study | |
| Fluoroquinolone | qnrA | F: GGGTATGGATATTATTGATAAAG R: CTAATCCGGCAGCACTATTTA |
50 | 670 | [24] |
| aac(6')-Ib-cr | F: GCGATGCTCTATGAGTGGCT R: TTTCTTCTTCCCACCGTCCG |
60 | 220 | This study | |
| Tetracycline | tet(A) | F: GGTTCACTCGAACGACGTCA R: CTGTCCGACAAGTTGCATGA |
57 | 577 | [26] |
| tet(B) | F: CCTCAGCTTCTCAACGCGTG R: GCACCTTGCTGATGACTCTT |
56 | 634 |