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. 2019 Jun 4;9(30):17501–17513. doi: 10.1039/c9ra02473e

Calibration curves and the contents of the identified polyphenols in FTE by HPLCa.

Peak no. t R (min) Identified/polyphenols Regression equation Y = aX + b R 2 Contentb (mg g−1)
1 6.43 Gallic acid Y = 5.9167X + 0.4124 0.9998 21.44 ± 0.09
2 7.16 Gallocatechin Y = 87.147X − 14.651 0.9992 26.56 ± 0.11
3 8.90 Epigallocatechin Y = 94.968X − 1.4862 0.9941 67.70 ± 0.79
4 9.39 Catechin Y = 21.611X + 3.4979 0.9995 10.48 ± 0.16
5 10.87 Caffeic acid Y = 23.684X + 8.7968 0.9992 22.27 ± 0.16
6 11.47 Epigallocatechin gallate Y = 8.7325X + 0.6203 0.9996 111.93 ± 0.06
7 12.06 Epicatechin Y = 7.898X + 0.0708 0.9994 6.12 ± 0.03
8 13.10 Gallocatechin gallate Y = 5.2995X + 3.1846 0.9999 3.79 ± 0.14
9 15.64 Epicatechin gallate Y = 4.5062X − 0.6542 0.9984 1.11 ± 0.01
10 23.87 Theaflavins Y = 23.073X + 3.0737 0.9937 4.84 ± 0.26
a

Y is the characteristic peak area in HPLC chromatograms, and X is the concentration of the samples.

b

Contents of each component in FTE is expressed as mg g−1 dried extract.