Calibration curves and the contents of the identified polyphenols in FTE by HPLCa.
Peak no. | t R (min) | Identified/polyphenols | Regression equation Y = aX + b | R 2 | Contentb (mg g−1) |
---|---|---|---|---|---|
1 | 6.43 | Gallic acid | Y = 5.9167X + 0.4124 | 0.9998 | 21.44 ± 0.09 |
2 | 7.16 | Gallocatechin | Y = 87.147X − 14.651 | 0.9992 | 26.56 ± 0.11 |
3 | 8.90 | Epigallocatechin | Y = 94.968X − 1.4862 | 0.9941 | 67.70 ± 0.79 |
4 | 9.39 | Catechin | Y = 21.611X + 3.4979 | 0.9995 | 10.48 ± 0.16 |
5 | 10.87 | Caffeic acid | Y = 23.684X + 8.7968 | 0.9992 | 22.27 ± 0.16 |
6 | 11.47 | Epigallocatechin gallate | Y = 8.7325X + 0.6203 | 0.9996 | 111.93 ± 0.06 |
7 | 12.06 | Epicatechin | Y = 7.898X + 0.0708 | 0.9994 | 6.12 ± 0.03 |
8 | 13.10 | Gallocatechin gallate | Y = 5.2995X + 3.1846 | 0.9999 | 3.79 ± 0.14 |
9 | 15.64 | Epicatechin gallate | Y = 4.5062X − 0.6542 | 0.9984 | 1.11 ± 0.01 |
10 | 23.87 | Theaflavins | Y = 23.073X + 3.0737 | 0.9937 | 4.84 ± 0.26 |
Y is the characteristic peak area in HPLC chromatograms, and X is the concentration of the samples.
Contents of each component in FTE is expressed as mg g−1 dried extract.