Fig. 9. Analysis of the mechanism of complex 1-mediated cytotoxicity in B16-F10 cells using Annexin/PI staining on a flow cytometer. (a) Control: no treatment, (b) cells treated with 10 μM of the complex and incubated for 24 h in the dark, (c) cells treated with 10 μM of the complex and irradiated for 35 min using the blue LED system at an output of 100 mW cm−2. FL1-H: Annexin staining, FL2-H: PI staining.
