LC-MS/MS conditions used for the analysis of [Ru(bipy)2(BC)]Cl2 (complex 1), [Ru(bipy)2(Phen)]Cl2 (complex 2), and the ligand BC.
| [Ru(bipy)2(BC)]Cl2 (complex 1) | [Ru(bipy)2(Phen)]Cl2 (complex 2) | BC | |
|---|---|---|---|
| Mobile phase | Isocratic 5% A: H2O (0.1% formic acid), 95% B: MeOH (0.1% formic acid), flow rate (0.5 mL min−1) | ||
| Precursor ion (m/z) | 386.86 | 296.78 | 361.16 |
| Product ions (m/z) | 308.7, 361.16 | 181.1, 218.67 | 345.23, 359.16 |
| Collision energy (V) | 27 | 27 | 52 |
| Retention time (min) | 0.40 | 0.38 | 0.50 |
| Mass mode | ESI positive mode | ESI positive mode | ESI positive mode |
| Scan type | Single reaction monitoring (SRM) | SRM | SRM |