. 2019 Jun 3;9(30):17254–17265. doi: 10.1039/c9ra02615k

IC₅₀ values (μM) of complexes 1 and 2 in dark versus light conditions and the calculated phototoxicity index (PI) on different cell lines. Cytotoxicity of BC, bipy, and cisplatin was assessed only in the dark and all values reported are the mean ± SEM of three separate experiments, each including samples in triplicates.

IC₅₀ values^a (μM)						log P
Treatment	A549	B16-F10	Caco-2	HT-29	MDA-MB-231	log P
Complex 1	Dark: 51.8^a,c ± 18.8	Dark: >100^a	Dark: 29.8^a ± 2.9	Dark: 91.0^a ± 5.90	Dark: 5.55^a ± 1.45	−1.57 ± 0.01
	Light: 4.96^a ± 1.16	Light: 0.11^b ± 0.10	Light: 14.8^a ± 0.81	Light: 12.0^b ± 1.9	Light: 0.63^a ± 0.90
	PI: 11.1	PI: >900	PI: 2.0	PI: 7.6	PI: 8.9
BC	>100^b	>100^a	>100^b	>100^c	>100^b	6.96^c
bipy	>100^b	>100	>100	>100^c	37.5^b ± 0.14	1.88^d
Complex 2^b	Dark: >100^b,c	Dark: >100^a	Dark: >100^b	Dark: >100^c	Dark: >100^b	−2.82 ± 0.10
	Light: >100^b,c	Light: >100^b	Light: >100^b	Light: >100^c	Light: >100^b
	PI: —	PI: —	PI: —	PI: —	PI: —
Cisplatin	7.74^b,c ± 1.00	7.70^a ± 0.20	21.8^b ± 0.54	>100^c	30.9^b ± 0.82	−2.21^c

Values in a column not sharing a common superscript a, b, or c are significantly different (p < 0.05).

IC₅₀ data previously reported by our group.¹³

log P values reported in the literature.^26,47

log P values estimated by ChemDraw Professional (v15.0, CambridgeSoft).