Skip to main content
. 2022 Apr 5;61(8):665–677. doi: 10.1021/acs.biochem.2c00080

Figure 2.

Figure 2

Top: EPR spectra of WT PHM mixed with oxygenated buffer at various time points; the purple trace shows fully oxidized, the black trace shows the ascorbate-reduced anaerobic enzyme, and the red trace shows a 120 s reoxidized enzyme. Bottom: quantitation via double integration (colors are the same as for spectra) and calibration against a standard solution of 300 μM Cu(II)–EDTA (blue). EPR conditions: frequency 9.63 GHz, T = 100 K, microwave power 20 mW, gain 10 dB, modulation amplitude 10 G, and sweep time 84 s.