Figure 4.

Product generated from the reaction of dansyl-YVG with the equimolar fully reduced enzyme chased with a 5-fold excess of unlabeled Ac-YVG. Blue circles represent the product generated with equimolar dansyl-YVG and the fully reduced enzyme; purple diamonds represent the product generated when the same experiment is conducted in the presence of a 5-fold molar excess of Ac-YVG. The black dashed line represents the expected dansyl product after chasing with Ac-YVG if multiple turnovers occur.