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. 2022 Apr 5;61(8):665–677. doi: 10.1021/acs.biochem.2c00080

Figure 5.

Figure 5

Ascorbate dependence of the rate of PHM reduction determined by stopped-flow using the Cu(II) PHM–azido adduct (λmax = 390 nm) as a reporter for the concentration of Cu(II)–enzyme remaining at each time point. Data are fit to a pre-equilibrium binding event with KD = 1.1 mM.