TABLE 3.
Composition of RP-HPLC fraction 12 of an endoproteinase Glu-C digest of CMPa
Observed mass (m/z) | Calculated mass (Da) | Assignmentb |
---|---|---|
2,282 | 2,279 | Ser(P)149 κ-casein-A(138–158)c |
2,350 | 2,348 | Ser(P)149 κ-casein-A(148–169) |
2,394 | 2,392 | Ser(P)149 κ-casein-B(148–169) |
Growth inhibition was 84% ± 9% (growth ± standard deviation [n = 3]) at a concentration of 0.14 mg/ml (determined by amino acid analysis).
Assigned by MS and N-terminal amino acid sequence analysis.
Amino acid sequence of Ser(P)149 κ-casein-A(138–158): AVESTVATLEDΣPEVIESPPE, where Σ is Ser(P).