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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: FEBS J. 2021 Dec 1;289(9):2613–2627. doi: 10.1111/febs.16291

Figure 3. Vitamin K-dependent carboxylation in UBIAD1-deficient HEK293 reporter cells supported by different forms of vitamin K.

Figure 3

(A and B) VKD carboxylation in UBIAD1-deficient FIXgla-PC/HEK293 reporter cells using (A) vitamin K1, MK-4, and vitamin K3 or (B) menaquinones as the substrates. Reporter cells were incubated with a cell culture medium containing increasing concentrations of K vitamins. Reporter-protein carboxylation was determined as described in the legend of Figure 1B. (C) The half-maximal stimulation concentration (EC50) of K vitamins as determined from Figure 3A and 3B using GraphPad software. Data are presented as mean ± SD.