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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: FEBS J. 2021 Dec 1;289(9):2613–2627. doi: 10.1111/febs.16291

Figure 5. Effect of UBIAD1 mutations on VKD carboxylation.

Figure 5

(A and B) UBIAD1 or its variants was transiently expressed in UBIAD1-deficient FIXgla-PC/HEK293 reporter cells. Transfected cells were incubated with a cell culture medium containing 1 μM vitamin K3 (A) or increasing concentrations of vitamin K3 (B) for 24 hours. Wild-type UBIAD1 activity was normalized to 100%. Data are presented as mean ± SD of three independent experiments (n=3). * T-test, P<0.001, compared with the wild-type UBIAD1 (WT). (C) The EC50 values of vitamin K3 for UBIAD1 variants were determined using GraphPad software. Data are presented as mean ± SD. * T-test, P<0.01, compared with the wild-type UBIAD1. (D) Protein expression levels of UBIAD1 variants and HMGCR were determined by western blot analysis. GAPDH was used as the loading control.