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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: FEBS J. 2021 Dec 1;289(9):2613–2627. doi: 10.1111/febs.16291

Figure 7. Effect of UBIAD1 mutations on GGPP recognition.

Figure 7

(A) UBIAD1 or the selected UBIAD1 mutants was transiently expressed in UBIAD1-deficient FIXgla-PC/HEK293 reporter cells. Transfected cells were incubated with a cell culture medium containing 10 μM atorvastatin, 1 μM vitamin K3, and increasing concentrations of GGPP for 24 hours. Reporter protein carboxylation was determined as described in the legend of Figure 1B. The EC50 values of GGPP for UBIAD1 variants were determined from Figure 7A using GraphPad software (B). Data are presented as mean ± SD. * T-test, P<0.01, compared with the wild-type UBIAD1 (WT).