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. Author manuscript; available in PMC: 2022 Nov 3.
Published in final edited form as: Cancer Immunol Res. 2022 May 3;10(5):656–669. doi: 10.1158/2326-6066.CIR-21-0170

Figure 1. Single-cell transcriptional data of NeuN tumors treated with HDACi entinostat alone or in combination with ICIs.

Figure 1.

A. Summary of cell numbers obtained for each treatment group in the NT2.5 tumor model (see Methods). Whole tumors from 20 mice, 4 in each experimental group, were used to perform scRNAseq. B. UMAP with each cell partition annotated by its corresponding cell type. C. UMAPS colored by canonical marker genes used for cell type identification. D. Dot plot showing top differentially expressed markers between partitions computed using monocle3. E. Cell weights for the CoGAPS NMF patterns found to correspond to partitions; boxplot lines correspond to medians. F. Gene weights for the genes most associated with each of the CoGAPS patterns that corresponded with cellular partitions. G. Proportion of cells in each group by treatment condition. H. Evaluation of Cd274 (PDL1) and Pdcd1lg2 (PDL2) ligand expression across cellular groups quantified in violin plot and overlayed with UMAP. White dots correspond to medians and black dots to means.